Coding

Part:BBa_K4286202:Design

Designed by: Bingru Feng   Group: iGEM22_SZU-China   (2022-08-25)


Prb1, a serine protease plays an important role in the destruction of plant pathogens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 250
    Illegal NgoMIV site found at 544
    Illegal NgoMIV site found at 658
    Illegal AgeI site found at 1174
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 391
    Illegal BsaI.rc site found at 268


Design Notes

The protease PRB1 has been proven to be an effective biocontrol agent against a variety of plant fungal pathogens. In our design, PRB1 will be overexpressed in Trichoderma atroviride to enhance its inhibitory effect on Rhizoctonia solani.


Source

PRB1 comes from Trichoderma atroviride IMI 206040.

References